Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)
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چکیده
منابع مشابه
Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy.
We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conj...
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We have previously reported (1) that the bifunctional reagent, p ,p~ difluoro-m ,rn p dinitrodiphenyl sulfone (FNPS) 1 can be used for conjugation of antibodies to ferritin in a one-step procedure, under conditions causing little loss of precipitating capacity of the antibody. In this brief report are discussed some details of these studies, the characterization of the antibodyferritin conjugat...
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Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, ...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2021
ISSN: 1439-4227,1439-7633
DOI: 10.1002/cbic.202100115